pkm2 overexpression vectors (Addgene inc)
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pkm2 overexpression vectors
Pkm2 Overexpression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkm2+overexpression+vectors/PKM2+(Plasmid+%2325538)/pmc08940925-184-3-17
Average 93 stars, based on 16 article reviews
Pkm2 Overexpression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkm2+overexpression+vectors/PKM2+(Plasmid+%2325538)/pmc08940925-184-3-17
Average 93 stars, based on 16 article reviews
pkm2 overexpression vectors - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis"
Article Title: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis
Journal: Cell Death & Disease
doi: 10.1038/s41419-022-04711-1
Figure Legend Snippet: A RT-PCR analysis on the correlation between YTHDF1 and PKM2 mRNA. B The m6A modification motif of PKM2 mRNA for YTHDF1 binding, analyzed by RMBase V2.0 database ( http://rna.sysu.edu.cn/rmbase/ ). C Breast cancer cell lines were co-transfected with siYTHDF1 and pmirGLO-PKM2 reporter for 48 h to determine the translation efficiency of PKM2 after different treatment, which was calculated by dividing protein yield with mRNA abundance (F-luc/R-luc). D YTHDF1 and PKM2 expression levels in breast cancer cells after the co-transfection with YTHDF1 siRNA and pCDNA3.1-PKM2-3×FLAG. E pH changes in the culture medium of breast cancer cells in different groups after 48 h. F , G The concentration of glucose and lactic acid in the culture medium of the breast cancer cells in different groups after 48 h. H Detection of YTHDF1 and PKM2 expression in the breast cancer cells after co-transfection with pCDNA3.1-YTHDF1-3×FLAG and PKM2 siRNA. I pH changes in the culture medium of breast cancer cells in different groups after 48 h. J , K The concentration of glucose and lactate in the culture medium of the breast cancer cells after 48 h. L Apoptosis levels of breast cancer cells after co-transfection with YTHDF1 siRNA and pCDNA3.1-PKM2-3×FLAG via FACS. M Apoptosis levels of breast cancer cells after co-transfection with pCDNA3.1-YTHDF1-3×FLAG and PKM2 siRNA via FACS. Statistical analysis results are presented as mean ± SEM, student’s t test, * P < 0.05, ** P < 0.01, *** P < 0.001.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Modification, Binding Assay, Transfection, Expressing, Cotransfection, Concentration Assay
Figure Legend Snippet: A , B Impact of YTHDF1 inhibition on the growth of subcutaneous tumors. C Western blot analysis regarding YTHDF1 and PKM2 expression in subcutaneous tumors at 28 days after transplantation. D , E Impact of YTHDF1 overexpression on the growth of subcutaneous tumors. F Western blot analysis regarding YTHDF1 and PKM2 expression in subcutaneous tumors. G , H Impact of agomir-16-5p transfection on the growth of subcutaneous tumors. I Western blot analysis regarding YTHDF1 and PKM2 expression in subcutaneous tumors at 24 days after agomir-16-5p treatment. J – L Immunohistochemical image regarding the lung metastasis of breast tumors in mice after YTHDF1 knockdown, YTHDF1 overexpression or agomir-16-5p treatment. M Immunohistochemical imaging of YTHDF1 and PKM2 in matched normal and cancerous tissues of breast cancer patients. N Schematic illustration showing YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis.
Techniques Used: Inhibition, Western Blot, Expressing, Transplantation Assay, Over Expression, Transfection, Immunohistochemical staining, Knockdown, Imaging
Related Articles
Over Expression:Article Title: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis Article Snippet: The plasmid for shYTHDF1 was constructed based on the pLKO.1 vector (Addgene, #8453) with the shYTHDF1 sequence (5′-GGACATTGGTACTTGGGATAA-3′), while mouse YTHDF1 overexpression plasmid was prepared by inserting YTHDF1 (NM_173761.3) into pLVX- puro vector (Addgene, #125839). .. Human YTHDF1 and Construct:Article Title: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis Article Snippet: The plasmid for shYTHDF1 was constructed based on the pLKO.1 vector (Addgene, #8453) with the shYTHDF1 sequence (5′-GGACATTGGTACTTGGGATAA-3′), while mouse YTHDF1 overexpression plasmid was prepared by inserting YTHDF1 (NM_173761.3) into pLVX- puro vector (Addgene, #125839). .. Human YTHDF1 and Plasmid Preparation:Article Title: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis Article Snippet: The plasmid for shYTHDF1 was constructed based on the pLKO.1 vector (Addgene, #8453) with the shYTHDF1 sequence (5′-GGACATTGGTACTTGGGATAA-3′), while mouse YTHDF1 overexpression plasmid was prepared by inserting YTHDF1 (NM_173761.3) into pLVX- puro vector (Addgene, #125839). .. Human YTHDF1 and Synthesized:Article Title: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis Article Snippet: The plasmid for shYTHDF1 was constructed based on the pLKO.1 vector (Addgene, #8453) with the shYTHDF1 sequence (5′-GGACATTGGTACTTGGGATAA-3′), while mouse YTHDF1 overexpression plasmid was prepared by inserting YTHDF1 (NM_173761.3) into pLVX- puro vector (Addgene, #125839). .. Human YTHDF1 and |